SARS-CoV-2 intra-host evolution during prolonged infection in an immunocompromised patient.
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ABSTRACT: Objectives
Intra-host SARS-CoV-2 evolution during chronic infection in immunocompromised hosts has been suggested as being the possible trigger of the emergence of new variants.Methods
Using a deep sequencing approach, we investigated the SARS-CoV-2 intra-host genetic evolution in a patient with HIV over a period of 109 days.Results
Sequencing of nasopharyngeal swabs at three time points demonstrated dynamic changes in the viral population, with the emergence of 26 amino acid mutations and two deletions, 57% of them in the Spike protein. Such a combination of mutations has never been observed in other SARS-CoV-2 lineages detected so far.Conclusion
Our data confirm that persistent infection in certain immunocompromised individuals for a long time may favor the dangerous emergence of new SARS-CoV-2 variants with immune evasion properties.
SUBMITTER: Quaranta EG
PROVIDER: S-EPMC9212919 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Publications
SARS-CoV-2 intra-host evolution during prolonged infection in an immunocompromised patient.
Quaranta Erika Giorgia EG Fusaro Alice A Giussani Edoardo E D'Amico Valeria V Varotto Maria M Pagliari Matteo M Giordani Maria Teresa MT Zoppelletto Maira M Merola Francesca F Antico Antonio A Stefanelli Paola P Terregino Calogero C Monne Isabella I
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 20220618
<h4>Objectives</h4>Intra-host SARS-CoV-2 evolution during chronic infection in immunocompromised hosts has been suggested as being the possible trigger of the emergence of new variants.<h4>Methods</h4>Using a deep sequencing approach, we investigated the SARS-CoV-2 intra-host genetic evolution in a patient with HIV over a period of 109 days.<h4>Results</h4>Sequencing of nasopharyngeal swabs at three time points demonstrated dynamic changes in the viral population, with the emergence of 26 amino ...[more]
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