Three-dimensional genome structure shapes the recombination landscape of chromatin features during female germline stem cell development.

ABSTRACT:

Background

During meiosis of mammalian cells, chromatin undergoes drastic reorganization. However, the dynamics of the three-dimensional (3D) chromatin structure during the development of female germline stem cells (FGSCs) are poorly understood.

Methods

The high-throughput chromosome conformation capture technique was used to probe the 3D structure of chromatin in mouse germ cells at each stage of FGSC development.

Results

The global 3D genome was dramatically reorganized during FGSC development. In topologically associating domains, the chromatin structure was weakened in germinal vesicle stage oocytes and still present in meiosis I stage oocytes but had vanished in meiosis II oocytes. This switch between topologically associating domains was related to the biologica

SUBMITTER: Tian GG 

PROVIDER: S-EPMC9214757 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature