Dataset Information

Genomic analysis of the first cases of extensively drug-resistant, travel-related Salmonella enterica serovar Typhi in Oman.

ABSTRACT:

Objectives

To highlight the importance of molecular testing in characterizing extensively drug-resistant (XDR) Salmonella Typhi (S. Typhi), and linking it to the current outbreak in Sindh, Pakistan.

Methods

Our study reports three travel-related typhoid fever cases caused by XDR S. Typhi that presented between January 2019 and August 2019. Antimicrobial susceptibility and genotyping with pulse-field gel electrophoresis (PFGE) were carried out. Whole-genome sequencing (WGS) was performed to characterize the genomic clonality in relation to the emerging outbreak of S. Typhi in Sindh, Pakistan, and to study the molecular resistance profiles.

Results

Laboratory testing revealed resistance to all first-line antibiotics (i.e ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole), as well as to quinolones and third-generation cephalosporins, leading to a change in the patients' therapy to the use of carbapenems. Classical MLST (cMLST) revealed that the strains were of sequence type 1 (ST1) and the core genome sequence (cgWGS) analysis closely clustered our strains with internationally reported strains from Pakistan, India, and the UK. The strains were found to carry a bla _CTX-15 gene-harbouring IncY plasmid, which encodes resistance to ceftriaxone.

Conclusions

Our report alerts clinicians to the use of appropriate empirical treatments in such scenarios, and highlights the significance of the global spread of XDR S. Typhi.

SUBMITTER: Al-Rashdi A

PROVIDER: S-EPMC9216381 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Publications

Genomic analysis of the first cases of extensively drug-resistant, travel-related <i>Salmonella enterica</i> serovar Typhi in Oman.

Al-Rashdi Azza A Kumar Rajesh R Al-Bulushi Mohamed M Abri Seif Al SA Al-Jardani Amina A

IJID regions 20211101

<h4>Objectives</h4>To highlight the importance of molecular testing in characterizing extensively drug-resistant (XDR) <i>Salmonella</i> Typhi (<i>S.</i> Typhi), and linking it to the current outbreak in Sindh, Pakistan.<h4>Methods</h4>Our study reports three travel-related typhoid fever cases caused by XDR <i>S.</i> Typhi that presented between January 2019 and August 2019. Antimicrobial susceptibility and genotyping with pulse-field gel electrophoresis (PFGE) were carried out. Whole-genome seq ...[more]

PMID: 35757821

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