Structural variants and modifications of hammerhead ribozymes targeting influenza A virus conserved structural motifs.

ABSTRACT: The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants of hammerhead ribozymes targeting conserved structural motifs of segment 5 of influenza A virus (IAV) (+)RNA. The variants carry structural and chemical modifications aiming to improve the RNA cleavage activity of ribozymes. We introduced an additional hairpin motif and attempted to select ribozyme-target pairs with sequence features that enable the potential formation of the trans-Hoogsteen interactions that are present in full-length, highly active hammerhead ribozymes. We placed structurally defined guanosine analogs into the ribozyme catalytic core. Herein, the significantly improved synthesis of 2'-deoxy-2'-fluoroarabinoguanosine deriva

SUBMITTER: Czapik T 

PROVIDER: S-EPMC9217987 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature