Efficient embryoid-based method to improve generation of optic vesicles from human induced pluripotent stem cells.

ABSTRACT: Animal models have provided many insights into ocular development and disease, but they remain suboptimal for understanding human oculogenesis. Eye development requires spatiotemporal gene expression patterns and disease phenotypes can differ significantly between humans and animal models, with patient-associated mutations causing embryonic lethality reported in some animal models. The emergence of human induced pluripotent stem cell (hiPSC) technology has provided a new resource for dissecting the complex nature of early eye morphogenesis through the generation of three-dimensional (3D) cellular models. By using patient-specific hiPSCs to generate in vitro optic vesicle-like models, we can enhance the understanding of early developmental eye disorders and provide a pre-clinical pla

SUBMITTER: Eintracht J 

PROVIDER: S-EPMC9218590 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature