Ontology highlight
ABSTRACT:
SUBMITTER: Senger M
PROVIDER: S-EPMC9219605 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Senger Moritz M Kernmayr Tobias T Lorenzi Marco M Redman Holly J HJ Berggren Gustav G
Chemical communications (Cambridge, England) 20220623 51
Small molecules in solution may interfere with mechanistic investigations, as they can affect the stability of catalytic states and produce off-cycle states that can be mistaken for catalytically relevant species. Here we show that the hydride state (H<sub>hyd</sub>), a proposed central intermediate in the catalytic cycle of [FeFe]-hydrogenase, can be formed in wild-type [FeFe]-hydrogenases treated with H<sub>2</sub> in absence of other, non-biological, reductants. Moreover, we reveal a new stat ...[more]