Dataset Information

Molecular Detection of Anaplasma phagocytophilum and Ehrlichia Species in Ticks Removed from Humans in the Republic of Korea.

ABSTRACT: Human granulocytic anaplasmosis (HGA) and human monocytic ehrlichiosis (HME) are zoonotic tick-borne diseases transmitted via tick bites. To determine the state of human Anaplasma and Ehrlichia infections caused by tick bites in the Republic of Korea (ROK), we conducted a nationwide investigation of human cases of tick bites in 2020. A total of 180 ticks were obtained, comprising Haemaphysalis longicornis (70.0%), Amblyomma testudinarium (17.8%), Ixodes nipponensis (6.1%), H. flava (4.4%), and I. persulcatus (1.7%). In three cases (1.7%; 95% CI: 0.3-4.9), A. phagocytophilum was detected in Ixodes ticks using primers for Anaplasma-specific genes (16s rRNA, ankA, and msp4). Conversely, Ehrlichia sp. was only detected in H. longicornis, in two cases (1.1%; 95% CI: 0.1-4.0). To the best of our knowledge, this is the first record of Ehrlichia sp. in ticks parasitizing humans in the ROK. As concerns remain about the possibility of HGA and HME transmission, continuous monitoring and management of the pathogens and vectors are necessary.

SUBMITTER: Kim YJ

PROVIDER: S-EPMC9227426 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Molecular Detection of Anaplasma phagocytophilum and Ehrlichia Species in Ticks Removed from Humans in the Republic of Korea.

Kim Yu-Jung YJ Seo Ji Ye JY Kim Seong Yoon SY Lee Hee Il HI

Microorganisms 20220615 6

Human granulocytic anaplasmosis (HGA) and human monocytic ehrlichiosis (HME) are zoonotic tick-borne diseases transmitted via tick bites. To determine the state of human Anaplasma and Ehrlichia infections caused by tick bites in the Republic of Korea (ROK), we conducted a nationwide investigation of human cases of tick bites in 2020. A total of 180 ticks were obtained, comprising Haemaphysalis longicornis (70.0%), Amblyomma testudinarium (17.8%), Ixodes nipponensis ...[more]

PMID: 35744742

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Project description:BackgroundAnaplasma spp., zoonotic tick-borne pathogens affecting livestock, companion animals, and humans, exhibits 15-18% seropositivity among hunting dogs in the Republic of Korea (South Korea). The dominant tick species in South Korea, Haemaphysalis longicornis, can transmit these pathogens to both humans and animals. Given the limited understanding of transovarial transmission of Anaplasma spp., our study aimed to assess the prevalence of questing larval ticks containing Anaplasma DNA. Additionally, we aimed to gather data for establishing a nationwide forecasting and alert system on seasonal variation of tick developmental stages and tick-borne zoonotic pathogens.MethodsFrom March to October 2021 and again from March to October 2022, we collected a total of 36,912 unfed, questing ticks of Haemaphysalis spp. from 149 sites in South Korea. Ticks were collected from herbaceous vegetation using the flagging method using a white flannel cloth. After species identification, one-third of collected ticks underwent analysis for Anaplasma DNA. Nymph ticks were pooled in groups of 1-10 and larvae in groups of 1-50, while adults were examined individually. Nested polymerase chain reaction (PCR) was performed to detect the genus Anaplasma by amplifying the 16S rRNA gene, followed by sequencing for species identification and phylogenetic analysis.ResultsOf the 36,912 questing ticks collected, 13,082 (35.4%) were identified as nymphs and adults of H. longicornis and 3850 (10.4%) as those of Haemaphysalis flava. The morphologically indistinguishable larval stage of Haemaphysalis spp. predominated, with 19,980 (54.1%) collected primarily from July to October. From the 939 tick pools, 24 pools (2.6%) tested positive for Anaplasma, with the larval stage exhibiting the highest number of positive pools (16, 1.7%). Phylogenetic analysis revealed that 21 of the 24 Anaplasma-positive pools contained A. phagocytophilum-specific genes, with 1 identified as Anaplasma sp. and the remaining 2 as A. bovis.ConclusionsOur study provides evidence of transovarial transmission of A. phagocytophilum in Haemaphysalis spp. larvae under field conditions, showing that the bacteria are transmitted from mother ticks to unengorged, questing larvae. Additionally, our findings contribute significant data for establishing a nationwide forecasting and alert system on seasonal variation of tick developmental stages and tick-borne zoonotic pathogens.

Dataset Information

Molecular Detection of Anaplasma phagocytophilum and Ehrlichia Species in Ticks Removed from Humans in the Republic of Korea.

Publications

Molecular Detection of <i>Anaplasma phagocytophilum</i> and <i>Ehrlichia</i> Species in Ticks Removed from Humans in the Republic of Korea.

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