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When to test for COVID-19 using RT-PCR: a systematic review.

ABSTRACT:

Objectives

The aim of this study was to evaluate the time in days between symptom onset and first positive RT-PCR result for COVID-19.

Methods

This systematic review was conducted in the MEDLINE (PubMed), Embase, and Scopus databases using the following descriptors: "COVID-19", "SARS-CoV-2", "coronavirus", "RT-PCR", "real time PCR" and "diagnosis".

Results

The included studies were conducted in 31 different countries and reported on a total of 6831 patients. The median age of the participants was 49.95 years. The three most common symptoms were fever, cough, and dyspnea, which affected 4012 (58.68%), 3192 (46.69%), and 2009 patients (29.38%), respectively. Among the 90 included studies, 13 were prospective cohorts, 15 were retrospective cohorts, 36 were case reports, 20 were case series, and 6 were cross-sectional studies. The overall mean time between symptom onset and positive test results was 6.72 days. Fourteen articles were analyzed separately for the temporal profile of RT-PCR tests, and the best performance was on days 22-24, when 98% of tests resulted positive.

Conclusions

These findings corroborate the RT-PCR COVID-19 testing practices of some health units. In addition, the most frequently described symptoms of these patients can be considered the initial symptoms of infection and used in decision-making about RT-PCR testing.

SUBMITTER: Dos Santos PG

PROVIDER: S-EPMC9233872 | biostudies-literature |

REPOSITORIES: biostudies-literature

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