Long-Read Metagenomics Improves the Recovery of Viral Diversity from Complex Natural Marine Samples.

ABSTRACT: The recovery of DNA from viromes is a major obstacle in the use of long-read sequencing to study their genomes. For this reason, the use of cellular metagenomes (>0.2-μm size range) emerges as an interesting complementary tool, since they contain large amounts of naturally amplified viral genomes from prelytic replication. We have applied second-generation (Illumina NextSeq; short reads) and third-generation (PacBio Sequel II; long reads) sequencing to compare the diversity and features of the viral community in a marine sample obtained from offshore waters of the western Mediterranean. We found that a major wedge of the expected marine viral diversity was directly recovered by the raw PacBio circular consensus sequencing (CCS) reads. More than 30,000 sequences were detected only in this d

SUBMITTER: Zaragoza-Solas A 

PROVIDER: S-EPMC9238414 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature