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Changes in the Abundance and Community Complexity of Soil Nematodes in Two Rice Cultivars Under Elevated Ozone.

ABSTRACT: The atmospheric ozone concentrations have substantially increased in the surface layer over the past decades, and consequently exhibited a strong influence on soil microbial communities and functions. However, the effect of elevated ozone (eO₃) on the abundance, diversity, and structural complexity of soil nematode communities are elusive under different rice (Oryza sativa L.) cultivars. Here, the soil nematode community was investigated in two rice cultivars (Hybrid, Shanyou 63 vs. Japonica, Wuyujing 3) under open-top chambers (OTC) with control and eO₃ conditions. The results showed that the abundance of soil nematode community was altered by eO₃, but the responses were dependent on crop cultivars. The eO₃ decreased the total abundance and simplified the network complexity of the soil nematode community for both cultivars. However, eO₃ increased the abundance of c-p 4 in Shanyou 63, rather than Wuyujing 3, indicating that the hybrid rice cultivar could tradeoff the adverse impacts of eO₃ on the functional group of soil nematodes. Similarly, bacterivores belonging to K-strategy (c-p 4) increased under eO₃ in Shanyou 63, suggesting that the soil food web formed a bacteria-dominated channel under eO₃ for the hybrid rice cultivar. This study shed new light on the critical importance of rice cultivars in shaping the impacts of eO₃ on the soil micro-food web. Therefore, breeding and biotechnological approaches may become valuable pathways to improve soil health by shaping the community structures of the soil micro-food web in response to climate change in the future.

SUBMITTER: Wang J

PROVIDER: S-EPMC9238508 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Microbes constitute a vital part of the plant holobiont. They establish plant-microbe or microbe-microbe associations, forming a unique microbiota with each plant species and under different environmental conditions. These microbial communities have to adapt to diverse environmental conditions, such as geographical location, climate conditions and soil types, and are subjected to changes in their surrounding environment. Elevated ozone concentration is one of the most important aspects of global change, but its effect on microbial communities living on plant surfaces has barely been investigated. In the current study, we aimed at elucidating the potential effect of elevated ozone concentrations on the phyllosphere (aerial part of the plant) and rhizoplane (surface of the root) microbiota by adopting next-generation 16S rRNA amplicon sequencing. A standard japonica rice cultivar Nipponbare and an ozone-tolerant breeding line L81 (Nipponbare background) were pre-grown in a greenhouse for 10 weeks and then exposed to ozone at 85 ppb for 7 h daily for 30 days in open top chambers. Microbial cells were collected from the phyllosphere and rhizoplane separately. The treatment or different genotypes did not affect various diversity indices. On the other hand, the relative abundance of some bacterial taxa were significantly affected in the rhizoplane community of ozone-treated plants. A significant effect of ozone was detected by homogeneity of molecular variance analysis in the phyllosphere, meaning that the community from ozone-treated phyllosphere samples was more variable than those from control plants. In addition, a weak treatment effect was observed by clustering samples based on the Yue and Clayton and weighted UniFrac distance matrices among samples. We therefore conclude that the elevated ozone concentrations affected the bacterial community structure of the phyllosphere and the rhizosplane as a whole, even though this effect was rather weak and did not lead to changes of the function of the communities.

Project description:Plant primary productivity and crop yields have been reduced due to the doubled level of global tropospheric ozone. Little is known about how elevated ozone affects soil microbial communities in the cropland ecosystem and whether such effects are sensitive to the nitrogen (N) supply. Here, we examined the responses of bacterial and fungal communities in maize soils to elevated ozone (+60 ppb ozone) across different levels of N fertilization (+60, +120, and +240 kg N ha-1yr-1). The fungal alpha diversity was decreased (P < 0.05), whereas the bacterial alpha diversity displayed no significant change under elevated ozone. Significant (P < 0.05) effects of N fertilization and elevated ozone on both the bacterial and fungal communities were observed. However, no interactive effects between N fertilization and elevated ozone were observed for bacterial and fungal communities (P > 0.1). The bacterial responses to N fertilization as well as the bacterial and fungal responses to elevated ozone were all phylogenetically conserved, showing universal homogeneous selection (homogeneous environmental conditions leading to more similar community structures). In detail, bacterial Alphaproteobacteria, Actinobacteria, and Chloroflexi, as well as fungal Ascomycota, were increased by elevated ozone, whereas bacterial Gammaproteobacteria, Bacteroidetes, and Elusimicrobia, as well as fungal Glomeromycota, were decreased by elevated ozone (P < 0.05). These ozone-responsive phyla were generally correlated (P < 0.05) with plant biomass, plant carbon (C) uptake, and soil dissolved organic C, demonstrating that elevated ozone affects plant-microbe interactions. Our study highlighted that microbial responses to elevated ozone display a phylogenetic clustering pattern, suggesting that response strategies to elevated ozone stress may be phylogenetically conserved ecological traits. IMPORTANCE The interactions of plant and soil microbial communities support plant growth and health. The increasing tropospheric ozone decreases crop biomass and also alters soil microbial communities, but the ways in which crops and their associated soil microbial communities respond to elevated tropospheric ozone are not clear, and it is also obscure whether the interactions between ozone and the commonly applied N fertilization exist. We showed that the microbial responses to both elevated ozone and N fertilization were phylogenetically conserved. However, the microbial communities that responded to N fertilization and elevated ozone were different, and this was further verified by the lack of an interactive effect between N fertilization and elevated ozone. Given that the global tropospheric ozone concentration will continue to increase in the coming decades, the decrease of specific microbial populations caused by elevated ozone would result in the extinction of certain microbial taxa. This ozone-induced effect will further harm crop production, and awareness is urgently needed.

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Changes in the Abundance and Community Complexity of Soil Nematodes in Two Rice Cultivars Under Elevated Ozone.

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