The Shigella Spp. Type III Effector Protein OspB Is a Cysteine Protease.

ABSTRACT: The type III secretion system is required for virulence of many pathogenic bacteria. Bacterial effector proteins delivered into target host cells by this system modulate host signaling pathways and processes in a manner that promotes infection. Here, we define the activity of the effector protein OspB of the human pathogen Shigella spp., the etiological agent of shigellosis and bacillary dysentery. Using the yeast Saccharomyces cerevisiae as a model organism, we show that OspB sensitizes cells to inhibition of TORC1, the central regulator of growth and metabolism. In silico analyses reveal that OspB bears structural homology to bacterial cysteine proteases that target mammalian cell processes, and we define a conserved cysteine-histidine catalytic dyad required for OspB funct

SUBMITTER: Wood TE 

PROVIDER: S-EPMC9239218 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature