Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system.
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ABSTRACT:
SUBMITTER: Fernandez LA
PROVIDER: S-EPMC92415 | biostudies-literature | 2000 Nov
REPOSITORIES: biostudies-literature
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Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system.
Fernández L A LA Sola I I Enjuanes L L de Lorenzo V V
Applied and environmental microbiology 20001101 11
A simple method for the nontoxic, specific, and efficient secretion of active single-chain Fv antibodies (scFvs) into the supernatants of Escherichia coli cultures is reported. The method is based on the well-characterized hemolysin transport system (Hly) of E. coli that specifically secretes the target protein from the bacterial cytoplasm into the extracellular medium without a periplasmic intermediate. The culture media that accumulate these Hly-secreted scFv's can be used in a variety of immu ...[more]
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