Spatial and temporal proteomics reveals the distinct distributions and dynamics of O-GlcNAcylated proteins.

ABSTRACT: Protein O-GlcNAcylation plays critical roles in many cellular events, and its dysregulation is related to multiple diseases. Integrating bioorthogonal chemistry and multiplexed proteomics, we systematically and site specifically study the distributions and dynamics of protein O-GlcNAcylation in the nucleus and the cytoplasm of human cells. The results demonstrate that O-GlcNAcylated proteins with different functions have distinct distribution patterns. The distributions vary site specifically, indicating that different glycoforms of the same protein may have different distributions. Moreover, we comprehensively analyze the dynamics of O-GlcNAcylated and non-modified proteins in these two compartments, respectively, and the half-lives of glycoproteins in different compartments are markedly

SUBMITTER: Xu S 

PROVIDER: S-EPMC9244862 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature