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Bitter taste signaling in tracheal epithelial brush cells elicits innate immune responses to bacterial infection.

ABSTRACT: Constant exposure of the airways to inhaled pathogens requires efficient early immune responses protecting against infections. How bacteria on the epithelial surface are detected and first-line protective mechanisms are initiated are not well understood. We have recently shown that tracheal brush cells (BCs) express functional taste receptors. Here we report that bitter taste signaling in murine BCs induces neurogenic inflammation. We demonstrate that BC signaling stimulates adjacent sensory nerve endings in the trachea to release the neuropeptides CGRP and substance P that mediate plasma extravasation, neutrophil recruitment, and diapedesis. Moreover, we show that bitter tasting quorum-sensing molecules from Pseudomonas aeruginosa activate tracheal BCs. BC signaling depends on the key taste transduction gene Trpm5, triggers secretion of immune mediators, among them the most abundant member of the complement system, and is needed to combat P. aeruginosa infections. Our data provide functional insight into first-line defense mechanisms against bacterial infections of the lung.

SUBMITTER: Hollenhorst MI

PROVIDER: S-EPMC9246383 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Bitter taste signaling in tracheal epithelial brush cells elicits innate immune responses to bacterial infection.

Hollenhorst Monika I MI Nandigama Rajender R Evers Saskia B SB Gamayun Igor I Abdel Wadood Noran N Salah Alaa A Pieper Mario M Wyatt Amanda A Stukalov Alexey A Gebhardt Anna A Nadolni Wiebke W Burow Wera W Herr Christian C Beisswenger Christoph C Kusumakshi Soumya S Ectors Fabien F Kichko Tatjana I TI Hübner Lisa L Reeh Peter P Munder Antje A Wienhold Sandra-Maria SM Witzenrath Martin M Bals Robert R Flockerzi Veit V Gudermann Thomas T Bischoff Markus M Lipp Peter P Zierler Susanna S Chubanov Vladimir V Pichlmair Andreas A König Peter P Boehm Ulrich U Krasteva-Christ Gabriela G

The Journal of clinical investigation 20220701 13

Constant exposure of the airways to inhaled pathogens requires efficient early immune responses protecting against infections. How bacteria on the epithelial surface are detected and first-line protective mechanisms are initiated are not well understood. We have recently shown that tracheal brush cells (BCs) express functional taste receptors. Here we report that bitter taste signaling in murine BCs induces neurogenic inflammation. We demonstrate that BC signaling stimulates adjacent sensory ner ...[more]

PMID: 35503420

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Project description:IntroductionBitter taste receptors (T2Rs) are G protein-coupled receptors identified on the tongue but expressed all over the body, including in airway cilia and macrophages, where T2Rs serve an immune role. T2R isoforms detect bitter metabolites (quinolones and acyl-homoserine lactones) secreted by gram negative bacteria, including Pseudomonas aeruginosa, a major pathogen in cystic fibrosis (CF). T2R activation by bitter bacterial products triggers calcium-dependent nitric oxide (NO) production. In airway cells, the NO increases mucociliary clearance and has direct antibacterial properties. In macrophages, the same pathway enhances phagocytosis. Because prior studies linked CF with reduced NO, we hypothesized that CF cells may have reduced T2R/NO responses, possibly contributing to reduced innate immunity in CF.MethodsImmunofluorescence, qPCR, and live cell imaging were used to measure T2R localization, calcium and NO signaling, ciliary beating, and antimicrobial responses in air-liquid interface cultures of primary human nasal epithelial cells and immortalized bronchial cell lines. Immunofluorescence and live cell imaging was used to measure T2R signaling and phagocytosis in primary human monocyte-derived macrophages.ResultsPrimary nasal epithelial cells from both CF and non-CF patients exhibited similar T2R expression, localization, and calcium signals. However, CF cells exhibited reduced NO production also observed in immortalized CFBE41o- CF cells and non-CF 16HBE cells CRISPR modified with CF-causing mutations in the CF transmembrane conductance regulator (CFTR). NO was restored by VX-770/VX-809 corrector/potentiator pre-treatment, suggesting reduced NO in CF cells is due to loss of CFTR function. In nasal cells, reduced NO correlated with reduced ciliary and antibacterial responses. In primary human macrophages, inhibition of CFTR reduced NO production and phagocytosis during T2R stimulation.ConclusionsTogether, these data suggest an intrinsic deficiency in T2R/NO signaling caused by loss of CFTR function that may contribute to intrinsic susceptibilities of CF patients to P. aeruginosa and other gram-negative bacteria that activate T2Rs.

Dataset Information

Bitter taste signaling in tracheal epithelial brush cells elicits innate immune responses to bacterial infection.

Publications

Bitter taste signaling in tracheal epithelial brush cells elicits innate immune responses to bacterial infection.

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