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Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites.


ABSTRACT: Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes.

SUBMITTER: Xiao L 

PROVIDER: S-EPMC92490 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature

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Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites.

Xiao L L   Limor J J   Morgan U M UM   Sulaiman I M IM   Thompson R C RC   Lal A A AA  

Applied and environmental microbiology 20001201 12


Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular diff  ...[more]

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