Unknown

Dataset Information

0

A commercial ARHGEF17/TEM4 antibody cross-reacts with Nuclear Mitotic Apparatus protein 1 (NuMA).

ABSTRACT: The Rho family Guanine nucleotide exchange factor (GEF) ARHGEF17 (also known as TEM4) is a large protein with only 3 annotated regions: an N-terminal actin-binding domain, a Rho-specific dbl homology (DH)- pleckstrin homology (PH) type GEF domain and a seven bladed β propeller fold at the C-terminus with unknown function. TEM4 has been implicated in numerous activities that rely on regulation of the cytoskeleton including cell migration, cell-cell junction formation and the spindle assembly checkpoint during mitosis. Here we have assessed the specificity of a TEM4 polyclonal antibody that has been commonly used as a Western blotting and immunocytochemistry probe for TEM4 in mammalian cells. We find that this antibody, in addition to its intended target, cross-reacts with the Nuclear Mitotic Apparatus Protein 1 (NuMA) in Western blotting and immunoprecipitation, and detects NuMA preferentially in immunocytochemistry. This cross-reactivity, with an abundant chromatin- and mitotic spindle-associated factor, is likely to affect the interpretation of experiments that make use of this antibody probe, in particular by immunocytochemistry and immunoprecipitation.

SUBMITTER: Prifti DK 

PROVIDER: S-EPMC9249204 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

A commercial ARHGEF17/TEM4 antibody cross-reacts with Nuclear Mitotic Apparatus protein 1 (NuMA).

Prifti Diogjena Katerina DK   Lauzier Annie A   Elowe Sabine S  

PloS one 20220701 7

The Rho family Guanine nucleotide exchange factor (GEF) ARHGEF17 (also known as TEM4) is a large protein with only 3 annotated regions: an N-terminal actin-binding domain, a Rho-specific dbl homology (DH)- pleckstrin homology (PH) type GEF domain and a seven bladed β propeller fold at the C-terminus with unknown function. TEM4 has been implicated in numerous activities that rely on regulation of the cytoskeleton including cell migration, cell-cell junction formation and the spindle assembly chec  ...[more]

Similar Datasets

Unknown A nonerythroid isoform of protein 4.1R interacts with the nuclear mitotic apparatus (NuMA) protein.
| S-EPMC2148212 | biostudies-literature
Unknown Resistance to inhibitors of cholinesterase 8A catalyzes release of Galphai-GTP and nuclear mitotic apparatus protein (NuMA) from NuMA/LGN/Galphai-GDP complexes.
| S-EPMC1283842 | biostudies-literature
Unknown The nuclear mitotic apparatus protein NuMA controls rDNA transcription and mediates the nucleolar stress response in a p53-independent manner.
| S-EPMC5714241 | biostudies-literature
Unknown Global Interactomics Connect Nuclear Mitotic Apparatus Protein NUMA1 to Influenza Virus Maturation.
| S-EPMC6316800 | biostudies-literature
Unknown A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component.
| S-EPMC7863998 | biostudies-literature
Transcriptomics A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB
2023-05-29 | GSE227893 | GEO
Unknown The Short Isoform of Nuclear Mitotic Apparatus Protein 1 Functions as a Putative Tumor Suppressor.
| S-EPMC5547835 | biostudies-literature
Unknown A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB.
| S-EPMC10259849 | biostudies-literature
Transcriptomics A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB [RIP-seq]
2023-05-29 | GSE227892 | GEO
Genomics A monoclonal antibody raised against human EZH2 cross-reacts with the RNA-binding protein SAFB [ChIP-seq]
2023-05-29 | GSE227890 | GEO