Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters.

ABSTRACT:

Background

Clubroot of canola (Brassica napus), caused by the soilborne pathogen Plasmodiophora brassicae, has become a serious threat to canola production in Canada. The deployment of clubroot-resistant (CR) cultivars is the most commonly used management strategy; however, the widespread cultivation of CR canola has resulted in the emergence of new pathotypes of P. brassicae capable of overcoming resistance. Several host differential sets have been reported for pathotype identification, but such testing is time-consuming, labor-intensive, and based on phenotypic classifications. The development of rapid and objective methods that allow for efficient, cost-effective and convenient pathotyping would enable testing of a much larger number of samples in shorter times. The aim of this

SUBMITTER: Tso HH 

PROVIDER: S-EPMC9250251 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature