Protocols for measuring cold-evoked neural activity and cold tolerance in Drosophila larvae following fictive cold acclimation.
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ABSTRACT:
SUBMITTER: Himmel NJ
PROVIDER: S-EPMC9253850 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
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Protocols for measuring cold-evoked neural activity and cold tolerance in Drosophila larvae following fictive cold acclimation.
Himmel Nathaniel J NJ Sakurai Akira A Donaldson Kevin J KJ Cox Daniel N DN
STAR protocols 20220629 3
Here, we outline protocols to study cold acclimation in Drosophila from a neurobiological perspective, starting with fictive cold acclimation using a custom-built optogenetics-housing apparatus we call the OptoBox. We also provide detailed steps for single-unit electrophysiological recordings from larval cold nociceptors and a high-throughput cold-tolerance assay. These protocols expand the toolkit for the study of insect cold acclimation and nociception. For complete details on the use and exec ...[more]
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