The dual effect of acetate on microglial TNF-α production.

ABSTRACT:

Introduction

Short-Chain Fatty Acids (SCFA) are products of intestinal microbial metabolism that can reach the brain and alter microglia in health and disease contexts. However, data are conflicting on the effect of acetate, the most abundant SCFA in the blood, in these cells.

Objective

The authors aimed to investigate acetate as a modulator of the inflammatory response in microglia stimulated with LPS.

Method

The authors used an immortalized cell line, C8-B4, and primary cells for in vitro treatments with acetate and LPS. Cell viability was analyzed by MTT, cytokine by RT-PCR, ELISA, and flow cytometry. The authors also performed in vivo and in silico analyses to study the role of acetate and the TNF-α contribution to the development of Experimental Autoimmune Enceph

SUBMITTER: Fragas MG 

PROVIDER: S-EPMC9254000 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature