Protocol for assessing ex vivo lipolysis of murine adipose tissue.
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ABSTRACT:
SUBMITTER: Roy D
PROVIDER: S-EPMC9254440 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Publications
Protocol for assessing ex vivo lipolysis of murine adipose tissue.
Roy Debasish D Myers Julia M JM Tedeschi Andrea A
STAR protocols 20220701 3
Here, we provide a detailed protocol for assessing ex vivo lipolysis of subcutaneous and visceral white adipose tissue. We describe a robust approach to detect depot-specific changes in lipolytic potential under basal and beta-adrenergic receptor-stimulated conditions. Given that adipose tissue plays a critical role in systemic metabolic health, this experimental protocol can be used to determine changes in adipose tissue function in health and disease. ...[more]
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