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Phospholipase C is a novel regulator at the early stages of microspore embryogenesis in Nicotiana tabacum.

ABSTRACT: Microspore transfers the developmental fate into embryogenesis in vitro regulated by determinant factors of stress-induced. However, the key regulators of microspore embryogenesis (ME) are still largely undiscovered to reveal the mechanism of cell fate transition. Here, we report that Phospholipase C (PLC) is involved at the early stages of ME in Nicotiana tabacum. NtPLC2/3/4 are expressed at the initial stages of ME. The expression levels of NtPLC2/3 are transient activated after 3 days in culture, while the expression level of NtPLC4 maintains relatively stable. Inhibition of PLCs induces the decrease in NtPLC2/3/4 expression level and decline of ME yield. We confirm that lipids in microspore are degraded and then re-accumulate at first embryonic division stage. Inhibition of PLCs suppresses the lipids metabolism at the early stages of ME. Thus, we propose that PLCs-mediated lipid metabolism is a novel regulator at the early stages of ME.

SUBMITTER: Luo P

PROVIDER: S-EPMC9254995 | biostudies-literature |

REPOSITORIES: biostudies-literature

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A novel late embryogenesis abundant like protein associated with chilling stress in Nicotiana tabacum cv. bright yellow-2 cell suspension culture.

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Project description: Not available

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Project description:Nicotiana tabacum L.

| PRJNA599461 | ENA

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De novo transcriptome assembly of transgenic tobacco (<i>Nicotiana tabacum</i> NC89) with early senescence characteristic.

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