Ontology highlight
ABSTRACT:
SUBMITTER: Keating JE
PROVIDER: S-EPMC9260395 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Keating James E JE Chung Chris C Chai Shengjie S Prins Jans F JF Vincent Benjamin G BG Hunsucker Sally A SA Armistead Paul M PM Glish Gary L GL
Journal of proteome research 20200720 8
Tandem mass spectrometry (MS/MS) is a highly sensitive and selective method for the detection of tumor-associated peptide antigens. These short, nontryptic sequences may lack basic residues, resulting in the formation of predominantly [peptide + H]<sup>+</sup> ions in electrospray. These singly charged ions tend to undergo inefficient dissociation, leading to issues in sequence determination. Addition of alkali metal salts to the electrospray solvent can drive the formation of [peptide + H + met ...[more]