Using population selection and sequencing to characterize natural variation of starvation resistance in Caenorhabditis elegans.

ABSTRACT: Starvation resistance is important to disease and fitness, but the genetic basis of its natural variation is unknown. Uncovering the genetic basis of complex, quantitative traits such as starvation resistance is technically challenging. We developed a synthetic-population (re)sequencing approach using molecular inversion probes (MIP-seq) to measure relative fitness during and after larval starvation in Caenorhabditis elegans. We applied this competitive assay to 100 genetically diverse, sequenced, wild strains, revealing natural variation in starvation resistance. We confirmed that the most starvation-resistant strains survive and recover from starvation better than the most starvation-sensitive strains using standard assays. We performed genome-wide association (GWA) with the MIP-s

SUBMITTER: Webster AK 

PROVIDER: S-EPMC9262388 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature