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Controllable assembly of synthetic constructs with programmable ternary DNA interaction.

ABSTRACT: Compared with the dual binding components in a binary interaction, the third component of a ternary interaction often serves as modulator or regulator in biochemical processes. Here, we presented a programmable ternary interaction strategy based on the natural DNA triplex structure. With the DNA triplex-based ternary interaction, we have successfully demonstrated controllable hierarchical assemblies from nanometer scale synthetic DNA nanostructure units to micrometer scale live bacteria. A selective signaling system responsive to orthogonal nucleic acid signals via ternary interaction was also demonstrated. This assembly method could further enrich the diversified design schemes of DNA nanotechnology.

SUBMITTER: Cui H

PROVIDER: S-EPMC9262601 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Controllable assembly of synthetic constructs with programmable ternary DNA interaction.

Cui Huangchen H Zhang Tianqing T Kong Yuhan Y Xing Hang H Wei Bryan B

Nucleic acids research 20220701 12

Compared with the dual binding components in a binary interaction, the third component of a ternary interaction often serves as modulator or regulator in biochemical processes. Here, we presented a programmable ternary interaction strategy based on the natural DNA triplex structure. With the DNA triplex-based ternary interaction, we have successfully demonstrated controllable hierarchical assemblies from nanometer scale synthetic DNA nanostructure units to micrometer scale live bacteria. A selec ...[more]

PMID: 35713533

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