Dataset Information

Phytochemical Investigation of Cordia africana Lam. Stem Bark: Molecular Simulation Approach.

ABSTRACT:

Background

The current work planned to evaluate Cordia africana Lam. stem bark, a traditionally used herb in curing of different ailments in Africa such as gastritis and wound infections, based on phytochemical and antibacterial studies of two pathogenic microorganisms: methicillin-resistant Staphylococcus aureus (MRSA) and Helicobacter pylori.

Methods

High performance liquid chromatography (HPLC) profiling was used for qualitative and quantitative investigation of the ethanol extract. The minimum inhibitory concentration (MIC) of the ethanolic extract and isolated compounds was estimated using the broth microdilution method and evidenced by molecular dynamics simulations.

Results

Four compounds were isolated and identified for the first time: α-amyrin, β-sitosterol, rosmarinic acid (RA) and methyl rosmarinate (MR). HPLC analysis illustrated that MR was the dominant phenolic acid. MR showed the best bacterial inhibitory activity against MRSA and H. pylori with MIC 7.81 ± 1.7 μg/mL and 31.25 ± 0.6, respectively, when compared to clarithromycin and vancomycin, respectively.

Conclusion

The antibacterial activity of the stem bark of Cordia africana Lam. was evidenced against MRSA and H. pylori. Computational modeling of the studied enzyme-ligands systems reveals that RA and MR can potentially inhibit both MRSA peptidoglycan transpeptidases and H. pylori urease, thereby creating a pathway via the use of a double target approach in antibacterial treatment.

SUBMITTER: Sabry MM

PROVIDER: S-EPMC9268672 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Publications

Phytochemical Investigation of <i>Cordia africana</i> Lam. Stem Bark: Molecular Simulation Approach.

Sabry Manal M MM El-Fishawy Ahlam M AM El-Rashedy Ahmed A AA El Gedaily Rania A RA

Molecules (Basel, Switzerland) 20220623 13

<h4>Background</h4>The current work planned to evaluate <i>Cordia africana</i> Lam. stem bark, a traditionally used herb in curing of different ailments in Africa such as gastritis and wound infections, based on phytochemical and antibacterial studies of two pathogenic microorganisms: methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Helicobacter pylori</i>.<h4>Methods</h4>High performance liquid chromatography (HPLC) profiling was used for qualitative and quantitative investigati ...[more]

PMID: 35807285

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