Hydroalcoholic extract of Haematoxylum brasiletto protects Caenorhabditis elegans from cadmium-induced toxicity

ABSTRACT:

Background

H. brasiletto is used in popular culture due to its therapeutic properties, including antioxidant, anti-inflammatory and antiproliferative properties, although little is known about its role as a protector against metal toxicity. This study aimed to investigate the chemical composition and efficacy of the hydroalcoholic extract from H. brasiletto (HAE-Hbrasiletto) collected in northern Colombia to defend against cadmium (Cd)-induced toxicity.

Methods

Phytochemical characterization was performed using HPLC-ESI-QTOF. Caenorhabditis elegans was employed to assess the shielding effect of HAE-Hbrasiletto against Cd toxicity in vivo, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was utilized to measure radical scavenging activity.

Results

The main secondary metabolites identified by HPLC-ESI-QTOF in the extracts were hematoxylins (brazilein and hematein) and protosappanins (protosappanin A, B and C, 10-O-methylprotosappanin B, and protosappanin A dimethyl acetal). The HAE-Hbrasiletto elicited low lethality in N2 worms and significantly reduced the Cd-induced death of the nematodes. It also improved Cd-induced motility inhibition, as well as body length and reproduction reduction provoked by the heavy metal. The extract displayed a good capacity to halt Cd-induced DAF-16 translocation. As this last process was associated with lethality (r = 0.962, p < 0.01), the antioxidant properties of the extract may contribute to ameliorating tissue damage induced by oxidative stress from Cd exposure.

Conclusion

HAE-Hbrasiletto has remarkable properties to protect against Cd-induced toxicity.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12906-022-03654-6. Highlights Most secondary metabolites tentatively identified in H. brasiletto are homoisoflavones. Hidroalcoholic extract of H. brasiletto protects C. elegans from Cd toxicity The extract diminished Cd-induced damage to reproduction, growth, and locomotion. Cd-induced oxidative stress and translocation of DAF-16 are blocked by the extract.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12906-022-03654-6.

SUBMITTER: Duran-Izquierdo M 

PROVIDER: S-EPMC9272861 | biostudies-literature |

REPOSITORIES: biostudies-literature