Dataset Information

Profiling vitamin D, its mediators and proinflammatory cytokines in rheumatoid arthritis: A case–control study

ABSTRACT: Abstract

Introduction

The active form of vitamin D has immunomodulatory and anti‐inflammatory effect. Vitamin D is implicated in pathogenesis of rheumatoid arthritis (RA) and its deficiency leads to increased inflammation. Moreover, its production is dependent on concentration of calcium, phosphorus, and parathyroid hormone (PTH). Cytokines mediates inflammation in RA synovium. This study evaluated vitamin D, its mediators and proinflammatory cytokines among RA patients.

Methods

In a case–control study, 78 RA patients from Komfo Anokye Teaching Hospital rheumatology clinic and 60 healthy blood donors were recruited. Chemistry analyzer and enzyme‐linked immunosorbent assay kits were used to measure biochemical parameters and cytokines.

Results

We found significantly higher levels of interleukin (IL)‐1β, interferon gamma (IFN‐γ), and tumor necrosis factor‐α (TNF‐α) in RA patients compared with controls (p < .05). There was a significant positive correlation between intact parathyroid hormone (iPTH) and IL‐10 (r = .30, p < .05) and a negative correlation between IL‐6 (r = −0.28, p > .05), IL‐1β (r = −0.25, p > .05), TNF‐α (r = −0.26, p > .05), IFN‐γ (r = −0.24, p > .05), and iPTH. There was a significant negative correlation between IL‐1β (r = −0.33, p < .05), IFN‐ γ (r = −0.29, p < .05), and calcium.

Conclusion

Reduced PTH, calcium, and phosphorus is associated with higher levels of proinflammatory cytokines which may worsen RA disease condition. Vitamin D is therefore not an independent regulator of proinflammatory cytokines in RA. The primary role of vitamin D has been established in mineral homeostasis, however, its production is dependent on serum concentration of calcium, phosphorus and parathyroid hormone. We found significantly higher levels of proinflammatory cytokines in RA patients compared with the healthy controls. A negative correlation was found between the proinflammatory cytokines (IL‐6, INF‐γ, IL‐1β, and TNF‐α) and the mediators of vitamin D (Ca, iPTH, and phosphorus); however, the anti‐inflammatory cytokine, IL‐10 correlated positively with the mediators of vitamin D.

SUBMITTER: Sakyi S

PROVIDER: S-EPMC9274797 | biostudies-literature |

REPOSITORIES: biostudies-literature

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