A Fluorometric CYP19A1 (Aromatase) Activity Assay in Live Cells.
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ABSTRACT:
SUBMITTER: Heidary DK
PROVIDER: S-EPMC9276262 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
A Fluorometric CYP19A1 (Aromatase) Activity Assay in Live Cells.
Heidary David K DK Kriger Sarah M SM Hachey Austin C AC Glazer Edith C EC
ChemMedChem 20210705 18
Inhibition of estrogen synthesis is an integral component of the frontline pharmacologic therapy for the treatment of estrogen receptor positive cancers. However, there is currently no direct, high-throughput-ready assay for aromatase (also known as CYP19A1) that can be performed in live cells. Herein we present a cell-based assay that allows for multiplexed assessment of enzyme activity, protein half-life, cell viability, and identification of inhibitors with slow off-rates. ...[more]
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