A novel nitidine chloride nanoparticle overcomes the stemness of CD133⁺EPCAM⁺ Huh7 hepatocellular carcinoma cells for liver cancer therapy.

ABSTRACT:

Background

Stemness of CD133⁺EPCAM⁺ hepatocellular carcinoma cells ensures cancer resistance to apoptosis,which is a challenge to current liver cancer treatments. In this study, we evaluated the tumorcidal activity of a novel nanoparticle of nitidine chloride (TPGS-FA/NC, TPGS-FA: folic acid modified D-α-tocopheryl polyethylene glycol 1000 succinate, NC: nitidine chloride), against human hepatocellular carcinoma (HCC) cell line Huh7 growth in vitro and in vivo.

Methods

Huh7 cells were treated with TPGS-FA/NC. Cell proliferation was assessed using MTT and colony assays. The expression of cell markers and signaling proteins was detected using western blot analyses. A sphere culture technique was used to enrich cancer stem cells (CSC) in Huh7 cells. TPGS-F

SUBMITTER: Li D 

PROVIDER: S-EPMC9277916 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature