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Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration.


ABSTRACT: Early diagnosis of articular cartilage damage and repeated evaluation of treatment efficacy are essential for osteoarthritis treatment. In this study, we established a simple ultrasound grading system for early degenerative articular cartilage and investigated its relationship with cartilage biological characteristics. The ultrasound grading system were based on surface integrity (S1a: continuous high-echo lines, S1b: discontinuous or weak high-echo lines, S2: surface irregular) and cartilage echogenicity (E1: with > 50%, E2: < 50% hypoechoic area of total cartilage layer) and verified by surface roughness (Ra; μm) and histological staining. Ra was lower in S1 than in S2, and the percentage of hypoechoic and safranin O-stained areas was positively correlated. Then we examined its relationship with histopathological evaluation (OARSI grade), gene expression, and protein production in responded to pro-inflammatory cytokine (IL-1ß) stimulation. OARSI grades were different among S grades. The superficial layer of S1 had higher expression of Collagen10, aggrecan, Sox9, and lower expression of Collagen1 and BMP2 than that of S2. S1 responded more pronouncedly to IL-1ß in IL-6, IL-8, and CCL2 production than S2. There was no difference among the E-grades. Taken together, our findings indicate that ultrasound assessment using surface integrity can reflect the biological characteristics of early degenerative articular cartilage.

SUBMITTER: Shi W 

PROVIDER: S-EPMC9279397 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Articular surface integrity assessed by ultrasound is associated with biological characteristics of articular cartilage in early-stage degeneration.

Shi Wen W   Kanamoto Takashi T   Aihara Masaharu M   Oka Shiro S   Kuroda Sanae S   Nakai Tsuyoshi T   Mazuka Takeo T   Takenaka Keisuke K   Sato Yuji Y   Tsukamoto Masahiro M   Ebina Kosuke K   Nakata Ken K  

Scientific reports 20220713 1


Early diagnosis of articular cartilage damage and repeated evaluation of treatment efficacy are essential for osteoarthritis treatment. In this study, we established a simple ultrasound grading system for early degenerative articular cartilage and investigated its relationship with cartilage biological characteristics. The ultrasound grading system were based on surface integrity (S1a: continuous high-echo lines, S1b: discontinuous or weak high-echo lines, S2: surface irregular) and cartilage ec  ...[more]

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