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Cloning and expression of a Ralstonia eutropha HF39 gene mediating indigo formation in Escherichia coli.

ABSTRACT: On complex medium Escherichia coli strains carrying hybrid plasmid pBEC/EE:11.0, pSKBEC/BE:9.0, pSKBEC/PP:3.3, or pSKBEC/PP:2.4 harboring genomic DNA of Ralstonia eutropha HF39 produced a blue pigment characterized as indigo by several chemical and spectroscopic methods. A 1,251-bp open reading frame (bec) was cloned and sequenced. The deduced amino acid sequence of bec showed only weak similarities to short-chain acyl-coenzyme A dehydrogenases, and the gene product catalyzed formation of indoxyl, a reactive preliminary stage for production of indigo.

SUBMITTER: Drewlo S 

PROVIDER: S-EPMC92822 | biostudies-literature | 2001 Apr

REPOSITORIES: biostudies-literature

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Cloning and expression of a Ralstonia eutropha HF39 gene mediating indigo formation in Escherichia coli.

Drewlo S S   Brämer C O CO   Madkour M M   Mayer F F   Steinbüchel A A  

Applied and environmental microbiology 20010401 4

On complex medium Escherichia coli strains carrying hybrid plasmid pBEC/EE:11.0, pSKBEC/BE:9.0, pSKBEC/PP:3.3, or pSKBEC/PP:2.4 harboring genomic DNA of Ralstonia eutropha HF39 produced a blue pigment characterized as indigo by several chemical and spectroscopic methods. A 1,251-bp open reading frame (bec) was cloned and sequenced. The deduced amino acid sequence of bec showed only weak similarities to short-chain acyl-coenzyme A dehydrogenases, and the gene product catalyzed formation of indoxy  ...[more]

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