Resolving isobaric interferences in direct infusion tandem mass spectrometry.

ABSTRACT:

Rationale

The co-fragmentation of precursors in direct infusion (DI) tandem high-resolution mass spectrometry (HRMS) can complicate the fragment spectra and consequently lead to false hits during compound identification.

Methods

The method herein described, termed IQAROS (incremental quadrupole acquisition to resolve overlapping spectra), modulates the intensities of precursors and fragments by stepwise movement of the quadrupole isolation window over the mass-to-charge (m/z) range of the precursors. The modulated signals are then deconvoluted by a linear regression model to reconstruct the fragment spectra with less interference. The hardware to demonstrate the use of IQAROS was an orbitrap with electrospray ionization (ESI) or secondary electrospray ionization (SESI), alth

SUBMITTER: Kaeslin J 

PROVIDER: S-EPMC9286799 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature