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Cell wall proteomic analysis of the cnidarian photosymbionts Breviolum minutum and Cladocopium goreaui.

ABSTRACT: The algal cell wall is an important cellular component that functions in defense, nutrient utilization, signaling, adhesion, and cell-cell recognition-processes important in the cnidarian-dinoflagellate symbiosis. The cell wall of symbiodiniacean dinoflagellates is not well characterized. Here, we present a method to isolate cell walls of Symbiodiniaceae and prepare cell-wall-enriched samples for proteomic analysis. Label-free liquid chromatography-electrospray ionization tandem mass spectrometry was used to explore the surface proteome of two Symbiodiniaceae species from the Great Barrier Reef: Breviolum minutum and Cladocopium goreaui. Transporters, hydrolases, translocases, and proteins involved in cell-adhesion and protein-protein interactions were identified, but the majority of cell wall proteins had no homologues in public databases. We propose roles for some of these proteins in the cnidarian-dinoflagellate symbiosis. This work provides the first proteomics investigation of cell wall proteins in the Symbiodiniaceae and represents a basis for future explorations of the roles of cell wall proteins in Symbiodiniaceae and other dinoflagellates.

SUBMITTER: Tortorelli G

PROVIDER: S-EPMC9293036 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Breviolum minutum

Project description:Symbiodinium minutum transcriptome assembly

| PRJNA274852 | ENA

Breviolum minutum

Project description:A endosymbiotic dinoflagellate within coral reefs

| PRJNA247417 | ENA

The proteome of dinoflagellate symbionts (Breviolum minutum) during symbiosis establishment in a model cnidarian

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2024-01-18 | PXD045585 | Pride

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Breviolum minutum strain:SSB01

Project description:Breviolum minutum strain:SSB01 Raw sequence reads

| PRJNA388224 | ENA

Breviolum minutum strain:SSB01

Project description:Breviolum minutum SSB01 Transcriptome

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