Unknown

Dataset Information

0

A Platform for Site-Specific DNA-Antibody Bioconjugation by Using Benzoylacrylic-Labelled Oligonucleotides.


ABSTRACT: Many bioconjugation strategies for DNA oligonucleotides and antibodies suffer limitations, such as site-specificity, stoichiometry and hydrolytic instability of the conjugates, which makes them unsuitable for biological applications. Here, we report a new platform for the preparation of DNA-antibody bioconjugates with a simple benzoylacrylic acid pentafluorophenyl ester reagent. Benzoylacrylic-labelled oligonucleotides prepared with this reagent can be site-specifically conjugated to a range of proteins and antibodies through accessible cysteine residues. The homogeneity of the prepared DNA-antibody bioconjugates was confirmed by a new LC-MS protocol and the bioconjugate probes were used in fluorescence or super-resolution microscopy cell imaging experiments. This work demonstrates the versatility and robustness of our bioconjugation protocol that gives site-specific, well-defined and plasma-stable DNA-antibody bioconjugates for biological applications.

SUBMITTER: Konc J 

PROVIDER: S-EPMC9297960 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7386253 | biostudies-literature
| S-EPMC6477694 | biostudies-literature
| S-EPMC7084160 | biostudies-literature
| S-EPMC7175205 | biostudies-literature
| S-EPMC7518637 | biostudies-literature
| S-EPMC3309776 | biostudies-literature
| S-EPMC7517114 | biostudies-literature
| S-EPMC5963889 | biostudies-literature
| S-EPMC1074722 | biostudies-literature
| S-EPMC6669078 | biostudies-literature