Genotyping of intraspecies polymorphisms of Sporothrix globosa using partial sequence of mitochondrial DNA.

ABSTRACT: Restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA) had been used for molecular identification of Sporothrix spp., which is the causative fungi of sporotrichosis and the most prevalent deep-seated dermatomycosis. Also, mtDNA-RFLP had been used to investigate the molecular epidemiology of sporotrichosis. While the current standard for molecular diagnosis is performed by sequence analysis of the calmodulin gene (CAL), correspondence between the results from CAL and mtDNA is of diagnostic and epidemiological interest. Here, we investigated the correspondence between CAL and mtDNA used for molecular identification of Sporothrix globosa and S. schenckii, which are two major species. We also investigated and propose molecular markers suitable to describe the epidemiology

SUBMITTER: Mochizuki H 

PROVIDER: S-EPMC9298766 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature