Profiling the specificity of clonally expanded plasma cells during chronic viral infection by single-cell analysis.

ABSTRACT: Plasma cells and their secreted antibodies play a central role in the long-term protection against chronic viral infection. However, due to experimental limitations, a comprehensive description of linked genotypic, phenotypic, and antibody repertoire features of plasma cells (gene expression, clonal frequency, virus specificity, and affinity) has been challenging to obtain. To address this, we performed single-cell transcriptome and antibody repertoire sequencing of the murine BM plasma cell population following chronic lymphocytic choriomeningitis virus infection. Our single-cell sequencing approach recovered full-length and paired heavy- and light-chain sequence information for thousands of plasma cells and enabled us to perform recombinant antibody expression and specificity screening.

SUBMITTER: Neumeier D 

PROVIDER: S-EPMC9299196 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature