Detection of clenbuterol residues in beef sausages and its enantiomeric analysis using UHPLC-MS/MS: A risk of unintentional doping in sport field.

ABSTRACT: Clenbuterol (Clb) can be present in Mexico often but not all over the world in animal tissues and organs, therefore, potentially is derived from animal sources as well. The aims of this study were to develop and validate a method for detecting traces of clenbuterol in beef sausages. A calibration curve showed linearity in the range of 20-500 pg ml^-1 . The limit of detection (LOD) and lower limit of quantification (LLOQ) were 5 and 10 pg g^-1 , respectively. The analyte recovery was from 95.70% to 100.40% with an intraday relative standard deviation (RSD%) of 0.99%-2.10% and an interday RSD% of 0.54%-2.34%, R²  = 0.9998. The methodology developed was applied successfully in 15 samples of beef sausage, and 73.3% of the samples tested contained racemic clenbute

SUBMITTER: Velasco-Bejarano B 

PROVIDER: S-EPMC9303807 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature