The pH-Dependence of the Hydration of 5-Formylcytosine: an Experimental and Theoretical Study.
Ontology highlight
ABSTRACT: 5-Formylcytosine is an important nucleobase in epigenetic regulation, whose hydrate form has been implicated in the formation of 5-carboxycytosine as well as oligonucleotide binding events. The hydrate content of 5-formylcytosine and its uracil derivative has now been quantified using a combination of NMR and mass spectroscopic measurements as well as theoretical studies. Small amounts of hydrate can be identified for the protonated form of 5-formylcytosine and for neutral 5-formyluracil. For neutral 5-formylcytosine, however, direct detection of the hydrate was not possible due to its very low abundance. This is in full agreement with theoretical estimates.
SUBMITTER: Zott FL
PROVIDER: S-EPMC9304204 | biostudies-literature |
REPOSITORIES: biostudies-literature
ACCESS DATA