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The pH-Dependence of the Hydration of 5-Formylcytosine: an Experimental and Theoretical Study.

ABSTRACT: 5-Formylcytosine is an important nucleobase in epigenetic regulation, whose hydrate form has been implicated in the formation of 5-carboxycytosine as well as oligonucleotide binding events. The hydrate content of 5-formylcytosine and its uracil derivative has now been quantified using a combination of NMR and mass spectroscopic measurements as well as theoretical studies. Small amounts of hydrate can be identified for the protonated form of 5-formylcytosine and for neutral 5-formyluracil. For neutral 5-formylcytosine, however, direct detection of the hydrate was not possible due to its very low abundance. This is in full agreement with theoretical estimates.

SUBMITTER: Zott FL

PROVIDER: S-EPMC9304204 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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The pH-Dependence of the Hydration of 5-Formylcytosine: an Experimental and Theoretical Study.

Zott Fabian L FL Korotenko Vasily V Zipse Hendrik H

Chembiochem : a European journal of chemical biology 20220210 7

5-Formylcytosine is an important nucleobase in epigenetic regulation, whose hydrate form has been implicated in the formation of 5-carboxycytosine as well as oligonucleotide binding events. The hydrate content of 5-formylcytosine and its uracil derivative has now been quantified using a combination of NMR and mass spectroscopic measurements as well as theoretical studies. Small amounts of hydrate can be identified for the protonated form of 5-formylcytosine and for neutral 5-formyluracil. For ne ...[more]

PMID: 35084086

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