A multispecies amplicon sequencing approach for genetic diversity assessments in grassland plant species.

ABSTRACT: Grasslands are widespread and economically relevant ecosystems at the basis of sustainable roughage production. Plant genetic diversity (PGD; i.e., within-species diversity) is related to many beneficial effects on the ecosystem functioning of grasslands. The monitoring of PGD in temperate grasslands is complicated by the multiplicity of species present and by a shortage of methods for large-scale assessments. However, the continuous advancement of high-throughput DNA sequencing approaches has improved the prospects of broad, multispecies PGD monitoring. Among them, amplicon sequencing stands out as a robust and cost-effective method. Here, we report a set of 12 multispecies primer pairs that can be used for high-throughput PGD assessments in multiple grassland plant species. The target lo

SUBMITTER: Loera-Sanchez M 

PROVIDER: S-EPMC9305562 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature