Combining a Genetically Engineered Oxidase with Hydrogen-Bonded Organic Frameworks (HOFs) for Highly Efficient Biocomposites.

ABSTRACT: Enzymes incorporated into hydrogen-bonded organic frameworks (HOFs) via bottom-up synthesis are promising biocomposites for applications in catalysis and sensing. Here, we explored synthetic incorporation of d-amino acid oxidase (DAAO) with the metal-free tetraamidine/tetracarboxylate-based BioHOF-1 in water. N-terminal enzyme fusion with the positively charged module Z_basic2 strongly boosted the loading (2.5-fold; ≈500 mg enzyme g_material ^-1 ) and the specific activity (6.5-fold; 23 U mg^-1 ). The DAAO@BioHOF-1 composites showed superior activity with respect to every reported carrier for the same enzyme and excellent stability during catalyst recycling. Further, extension to other enzymes, including cytochrome P450 BM3 (used in the production of

SUBMITTER: Wied P 

PROVIDER: S-EPMC9305891 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature