Unknown

Dataset Information

0

Extensive co-binding and rapid redistribution of NANOG and GATA6 during emergence of divergent lineages.

ABSTRACT: Fate-determining transcription factors (TFs) can promote lineage-restricted transcriptional programs from common progenitor states. The inner cell mass (ICM) of mouse blastocysts co-expresses the TFs NANOG and GATA6, which drive the bifurcation of the ICM into either the epiblast (Epi) or the primitive endoderm (PrE), respectively. Here, we induce GATA6 in embryonic stem cells-that also express NANOG-to characterize how a state of co-expression of opposing TFs resolves into divergent lineages. Surprisingly, we find that GATA6 and NANOG co-bind at the vast majority of Epi and PrE enhancers, a phenomenon we also observe in blastocysts. The co-bound state is followed by eviction and repression of Epi TFs, and quick remodeling of chromatin and enhancer-promoter contacts thus establishing the PrE lineage while repressing the Epi fate. We propose that co-binding of GATA6 and NANOG at shared enhancers maintains ICM plasticity and promotes the rapid establishment of Epi- and PrE-specific transcriptional programs.

SUBMITTER: Thompson JJ 

PROVIDER: S-EPMC9308780 | biostudies-literature |

REPOSITORIES: biostudies-literature

Json Xml

Similar Datasets

Transcriptomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates
2021-07-29 | GSE181104 | GEO
Other Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [Hi-C]
2021-07-30 | GSE181103 | GEO
Genomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [ChIP-seq]
2021-07-30 | GSE181097 | GEO
Genomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [ATAC-seq]
2021-07-30 | GSE181073 | GEO
Transcriptomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [RNA-seq]
2021-07-30 | GSE181072 | GEO
Genomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [CUT&TAG, CUT&RUN]
2021-07-30 | GSE181100 | GEO
Genomics Rapid redistribution and extensive cobinding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [reChIP]
2022-01-16 | GSE193738 | GEO
Genomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates
| PRJNA750714 | ENA
Genomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [ATAC-seq]
| PRJNA750562 | ENA
Genomics Rapid redistribution and extensive co-binding of NANOG and GATA6 at shared regulatory elements underlie specification of divergent cell fates [RNA-seq]
| PRJNA750527 | ENA