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Development of a highly efficient prime editor 2 system in plants.

ABSTRACT: Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T₀ rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.

SUBMITTER: Li J

PROVIDER: S-EPMC9310484 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Easy-Prime: a machine learning-based prime editor design tool.

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