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Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion.

ABSTRACT: Here we demonstrate a switchable DNA electron-transfer catalyst, enabled by selective destabilization of secondary structure by the denaturant, perchlorate. The system is comprised of two strands, one of which can be selectively switched between a G-quadruplex and duplex or single-stranded conformations. In the G-quadruplex state, it binds hemin, enabling peroxidase activity. This switching ability arises from our finding that perchlorate, a chaotropic Hofmeister ion, selectively destabilizes duplex over G-quadruplex DNA. By varying perchlorate concentration, we show that the DNA structure can be switched between states that do and do not catalyze electron-transfer catalysis. State switching can be achieved in three ways: thermally, by dilution, or by concentration.

SUBMITTER: Hoog TG

PROVIDER: S-EPMC9310614 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion.

Hoog Tanner G TG Pawlak Matthew R MR Aufdembrink Lauren M LM Bachan Benjamin R BR Galles Matthew B MB Bense Nicholas B NB Adamala Katarzyna P KP Engelhart Aaron E AE

Chembiochem : a European journal of chemical biology 20220323 9

Here we demonstrate a switchable DNA electron-transfer catalyst, enabled by selective destabilization of secondary structure by the denaturant, perchlorate. The system is comprised of two strands, one of which can be selectively switched between a G-quadruplex and duplex or single-stranded conformations. In the G-quadruplex state, it binds hemin, enabling peroxidase activity. This switching ability arises from our finding that perchlorate, a chaotropic Hofmeister ion, selectively destabilizes du ...[more]

PMID: 35245408

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