Photothermal Regulated Nanozyme of CuFeS₂ Nanoparticles for Efficiently Promoting Wound Healing Infected by Multidrug Resistant Bacteria.

ABSTRACT: Peroxidase-mediated chemokinetic therapy (CDT) can effectively resist bacteria; however, factors such as the high dosage of drugs seriously limit the antibacterial effect. Herein, CuFeS₂ nanoparticles (NPs) nanozyme antibacterial system with the photothermal effect and peroxidase-like catalytic activity are proposed as a combined antibacterial agent with biosafety, high-efficiency, and broad-spectrum antibacterial ability. In addition, the as-obtained CuFeS₂ NPs with a low doses of Cu⁺ and Fe³⁺ can change the permeability of bacterial cell membranes and break the antioxidant balance by consuming intracellular glutathione (GSH), which results in more conducive ROS production. Meanwhile, the photothermal heating can regulate the CuFeS₂ NPs close to their optimal reaction temperature (60 °C) to release more hydroxyl radical in low concentrations of H₂O₂ (100 µM). The proposed CuFeS₂ NPs-based antibacterial system achieve more than 99% inactivation efficiency of methicillin-resistant Staphylococcus aureus (10⁶ CFU mL^-1 MRSA), hyperspectral bacteria β-Escherichia coli (10⁶ CFU mL^-1 ESBL) and Pseudomonas aeruginosa (10⁶ CFU mL^-1 PA), even at low concentration (2 μg mL^-1), which is superior to those of the conventional CuO NPs at 4 mg mL^-1 reported in the literature. In vivo experiments further confirm that CuFeS₂ NPs can effectively treat wounds infected by MRSA and promote the wound healing. This study demonstrates that excellent antibacterial ability and good biocompatibility make CuFeS₂ NPs a potential anti-infection nanozyme with broad application prospects.

SUBMITTER: Liu Z 

PROVIDER: S-EPMC9315979 | biostudies-literature |

REPOSITORIES: biostudies-literature