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Diversity and distribution in hypersaline microbial mats of bacteria related to Chloroflexus spp.


ABSTRACT: Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern in Guerrero Negro, Mexico, were subjected to cloning or denaturing gradient gel electrophoresis and then sequenced. We found evidence of a high diversity of bacteria related to Chloroflexus which exhibit different distributions along a gradient of salinity from 5.5 to 16%.

SUBMITTER: Nubel U 

PROVIDER: S-EPMC93173 | biostudies-literature | 2001 Sep

REPOSITORIES: biostudies-literature

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Diversity and distribution in hypersaline microbial mats of bacteria related to Chloroflexus spp.

Nübel U U   Bateson M M MM   Madigan M T MT   Kühl M M   Ward D M DM  

Applied and environmental microbiology 20010901 9


Filamentous bacteria containing bacteriochlorophylls c and a were enriched from hypersaline microbial mats. Based on phylogenetic analyses of 16S rRNA gene sequences, these organisms form a previously undescribed lineage distantly related to Chloroflexus spp. We developed and tested a set of PCR primers for the specific amplification of 16S rRNA genes from filamentous phototrophic bacteria within the kingdom of "green nonsulfur bacteria." PCR products recovered from microbial mats in a saltern i  ...[more]

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