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Aerobic Degradation Characteristics of Decabromodiphenyl ether through Rhodococcus ruber TAW-CT127 and Its Preliminary Genome Analysis.


ABSTRACT: Decabromodiphenyl ether (BDE-209), a polybrominated diphenyl ether (PBDE) homolog, seriously threatens human health. In this study, a Rhodococcus ruber strain with high BDE-209 degradation activity, named TAW-CT127, was isolated from Tong'an Bay, Xiamen. Under laboratory conditions, the strain's optimal growth temperature, pH, and salinity are 45 °C, 7.0, and 0-2.5%, respectively. Scanning electron microscopy (SEM) analysis shows that TAW-CT127 is damaged when grown in manual marine culture (MMC) medium with BDE-209 as the sole carbon source instead of eutrophic conditions. In the dark, under the conditions of 28 °C, 160 rpm, and 3 g/L (wet weight) TAW-CT127, the degradation rate of 50 mg/L BDE-209 is 81.07%. The intermediate metabolites are hexabromo-, octabromo-, and nonabromo-diphenyl ethers. Through whole-genome sequencing, multiple dehalogenases were found in the genome of TAW-CT127; these may be involved in the production of lower-brominated diphenyl ethers. Additionally, biphenyl-2,3-dioxygenase (BDO) in TAW-CT127 may catalyze the debromination reaction of BDE-209. Our research provides a new high-efficiency strain for bioremediation of BDE-209 pollution, and lays the foundation for the preliminary exploration of genes associated with BDE-209 degradation.

SUBMITTER: Xu H 

PROVIDER: S-EPMC9319644 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature

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Aerobic Degradation Characteristics of Decabromodiphenyl ether through <i>Rhodococcus ruber</i> TAW-CT127 and Its Preliminary Genome Analysis.

Xu Hao H   Cai Qingtao Q   An Qiuying Q   Tang Chen C   Wang Wanpeng W   Wang Guangshun G   You Wanting W   Guo Dongbei D   Zhao Ran R  

Microorganisms 20220717 7


Decabromodiphenyl ether (BDE-209), a polybrominated diphenyl ether (PBDE) homolog, seriously threatens human health. In this study, a <i>Rhodococcus ruber</i> strain with high BDE-209 degradation activity, named TAW-CT127, was isolated from Tong'an Bay, Xiamen. Under laboratory conditions, the strain's optimal growth temperature, pH, and salinity are 45 °C, 7.0, and 0-2.5%, respectively. Scanning electron microscopy (SEM) analysis shows that TAW-CT127 is damaged when grown in manual marine cultu  ...[more]

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