Characterization and Comparison of Ocular Surface Microbiome in Newborns.
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ABSTRACT: Methods
A total of 20 conjunctival swabs were collected from newborns at birth and after antibiotic treatment. Samples were subjected to 16S rRNA sequencing via system MiSeq Illumina. The data were processed with the MicrobAT software and statistical analysis were performed using two-way ANOVA.Results
Antibiotic prophylaxis with gentamicin altered the composition of the microbiota. In detail, a 1.5- and 2.01-fold reduction was recorded for Cutibacterium acnes (C. acnes) and Massilia timonae (M. timonae), respectively, whereas an increase in Staphylococcus spp. of 6.5 times occurred after antibiotic exposure.Conclusions
Antibiotic prophylaxis altered the ocular microbiota whose understanding could avoid adverse effects on eye health.
SUBMITTER: Petrillo F
PROVIDER: S-EPMC9320102 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Publications
Characterization and Comparison of Ocular Surface Microbiome in Newborns.
Petrillo Francesco F Petrillo Arianna A Marrapodi Maddalena M Capristo Carlo C Gicchino Maria Francesca MF Montaldo Paolo P Caredda Elisabetta E Reibaldi Michele M Boatti Lara M V LMV Dell'Annunziata Federica F Folliero Veronica V Galdiero Marilena M
Microorganisms 20220710 7
The ocular microbiome is of fundamental importance for immune eye homeostasis, and its alteration would lead to an impairment of ocular functionality. Little evidence is reported on the composition of the ocular microbiota of term infants and on the impact of antibiotic prophylaxis.<h4>Methods</h4>A total of 20 conjunctival swabs were collected from newborns at birth and after antibiotic treatment. Samples were subjected to 16S rRNA sequencing via system MiSeq Illumina. The data were processed w ...[more]
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