Identifying Endogenous Cellular Proteins Destabilizing the Propagation of Swi1 Prion upon Overproduction.

ABSTRACT: (1) Background: Numerous prions exist in the budding yeast, including [SWI⁺], the prion form of Swi1-a subunit of the chromatin-remodeling complex SWI/SNF. Despite decades of research, the molecular mechanisms underlying prion initiation and propagation are not fully understood. In this study, we aimed to identify endogenous cellular proteins that destabilize [SWI⁺]. (2) Methods: We screened the MoBY-ORF 2.0 library for proteins that destabilize [SWI⁺] upon overproduction. We further explored the effects of the identified candidates against other yeast prions and analyzed their potential prion-curing mechanisms. (3) Results: Eighty-two [SWI⁺] suppressors were identified, and their effects were shown to be [SWI

SUBMITTER: Du Z 

PROVIDER: S-EPMC9321512 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature