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Microbial community development during syngas methanation in a trickle bed reactor with various nutrient sources.

ABSTRACT: Microbial community development within an anaerobic trickle bed reactor (TBR) during methanation of syngas (56% H₂, 30% CO, 14% CO₂) was investigated using three different nutrient media: defined nutrient medium (241 days), diluted digestate from a thermophilic co-digestion plant operating with food waste (200 days) and reject water from dewatered digested sewage sludge at a wastewater treatment plant (220 days). Different TBR operating periods showed slightly different performance that was not clearly linked to the nutrient medium, as all proved suitable for the methanation process. During operation, maximum syngas load was 5.33 L per L packed bed volume (pbv) & day and methane (CH₄) production was 1.26 L CH₄/L_pbv/d. Microbial community analysis with Illumina Miseq targeting 16S rDNA revealed high relative abundance (20-40%) of several potential syngas and acetate consumers within the genera Sporomusa, Spirochaetaceae, Rikenellaceae and Acetobacterium during the process. These were the dominant taxa except in a period with high flow rate of digestate from the food waste plant. The dominant methanogen in all periods was a member of the genus Methanobacterium, while Methanosarcina was also observed in the carrier community. As in reactor effluent, the dominant bacterial genus in the carrier was Sporomusa. These results show that syngas methanation in TBR can proceed well with different nutrient sources, including undefined medium of different origins. Moreover, the dominant syngas community remained the same over time even when non-sterilised digestates were used as nutrient medium. KEY POINTS: • Independent of nutrient source, syngas methanation above 1 L/L_pbv/D was achieved. • Methanobacterium and Sporomusa were dominant genera throughout the process. • Acetate conversion proceeded via both methanogenesis and syntrophic acetate oxidation.

SUBMITTER: Cheng G

PROVIDER: S-EPMC9329420 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:At present, a few chemicals can be separated after further processing of high-temperature coal tar (HTCT) distillates, which have a lower utilization. However, hydrogenation to produce clean fuel oil has not been widely reported in literature. Thus, due to the use of new feedstocks and the implementation of more severe environmental legislations, deep hydrodesulfurization (HDS) of HTCT will face formidable challenges. A series of HDS experiments were performed in a continuous isothermal trickle bed reactor in which the reactor temperature was varied from 648 to 678 K, the pressure from 12 to 16 MPa, and the liquid hourly space velocity (LHSV) from 0.25 to 0.35 h-1, and hydrogen-to-oil ratio kept constant at 2000 L/L. Based on the experimental data, possible reaction pathways of HDS reaction were investigated, and a modified Langmuir-Hinshelwood (LH) HTCT desulfurization kinetic model was established. gPROMS software was used to obtain optimal kinetic parameters that are as follows: EA = 26,842, K 0 = 93,958, ? = -1.14, n = 1.65, and m = 0.86. The model can well reproduce various working conditions and has better prediction accuracy. Some characteristics of HTCT HDS reactions were discovered; the reaction order (n) of HTCT HDS is slightly higher than that of crude oil and medium/low-temperature coal tar (M/LTCT), but the activation energy (EA) is relatively smaller. The established reactor model was used to predict the changes of the concentration of hydrogen, hydrogen sulfide, and sulfur compounds in the gas, liquid, and solid phases along the length of the reactor, respectively. The model was also used to predict the effects of pressure, temperature, and LHSV on the conversion rate of sulfur and catalyst effectiveness factors. The results showed that the LHSV has a greater impact on the conversion rate, and the pressure and temperature are less pronounced at high-severity operating conditions; the effectiveness factor is significantly smaller than that of other HDS processes, temperature has a greater effect on the effectiveness factor, followed by pressure and LHSV. The conclusion can provide a basis for further understanding the HTCT hydrotreating process.

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Microbial community development during syngas methanation in a trickle bed reactor with various nutrient sources.

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