Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.
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ABSTRACT:
SUBMITTER: Higgins JA
PROVIDER: S-EPMC93307 | biostudies-literature | 2001 Nov
REPOSITORIES: biostudies-literature
Publications
Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.
Higgins J A JA Jenkins M C MC Shelton D R DR Fayer R R Karns J S JS
Applied and environmental microbiology 20011101 11
The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum. ...[more]
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