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Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.

ABSTRACT: The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum.

SUBMITTER: Higgins JA 

PROVIDER: S-EPMC93307 | biostudies-literature | 2001 Nov

REPOSITORIES: biostudies-literature

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Rapid extraction of DNA From Escherichia coli and Cryptosporidium parvum for use in PCR.

Higgins J A JA   Jenkins M C MC   Shelton D R DR   Fayer R R   Karns J S JS  

Applied and environmental microbiology 20011101 11

The Xtra Amp tube, Isocode paper, Instagene matrix, and PrepMan matrix methods were evaluated for their ability to rapidly extract PCR-quality DNAs from Escherichia coli O157:H7 and Cryptosporidium parvum. All methods provided satisfactory DNA from E. coli, and the Xtra Amp and Instagene reagents provided satisfactory DNA from C. parvum. ...[more]

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